What is PCR?Polymerase Chain Reaction (PCR) PCR is a laboratory method used for making a very large number of copies of short sections of DNA from a very small sample of genetic material. This process is called "amplifying" the DNA and it enables specific genes of interest to be detected or measured.
What is Real Time PCR?Real-time PCR can detect the amplification of products, as the products are synthesized. The real-time PCR uses a florescent dye system and thermocycler equipped with fluorescent- detection capability.
What is Diffrence Between Conventional PCR and Real Time PCR?
- Conventional PCR is more time consuming as it uses gel electrophoresis to analyze the amplified PCR products Where Real-time PCR uses fluorescent dye system and less time consuming as it can detect early phases of the reaction.
- Real-time PCR is more sensitive than conventional PCR
- Conventional PCR has very poor resolution while real-time PCR can detect very little changes due to the high resolution
- Unlike conventional PCR, automated detection techniques are found in real-time PCR
- Unlike real-time PCR, conventional PCR cannot differentiate between dead and live bacteria
Features of Real Time PCR for TB Diagnosis:
1. Sensitive Technique: Highly sensitive technique & can detect as few as 1-2 mycobacteria in specimen.
2. Early Diagnosis: Found to be useful in diagnosing early disease as well as confirming diagnosis in clinically suspected cases.
3. Validated for PTB & EPTB: Most useful test for diagnosis of paucibacillary form of pulmonary or extra pulmonary or extra pulmonary infections by MTC.
4. Highly Efficient: Differentiate between typical & atypical mycobacteria (MTC & MOTT).
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