Skip to main content

TB Diagnosis: Line Probe Assay/ Hains Test

Introduction:

LPA(Line Probe Assay) or Hains test/ Molecular Methods is used to diagnose MDR/XDR TB Rapidly. This test is endorsed by WHO and FIND(Foundation for innovative new diagnostics.

Line Probe Assay Steps: 

  1.  DNA is extracted from M. tuberculosis isolates or directly from clinical specimens.
  2.  Polymerase chain reaction (PCR) amplification of the resistance-determining region of the gene under question is performed using biotinylated primers.

Features of LPA or Hains Test:

1. Rapid genotypic test based on DNA strip technology

2. Rapid dterminationof genetic mutatins associated with spcific 1st line & 2nd line drugs.

3. Line Probe Assay (LPA) technology is suitable for use at national/central reference laboratories, or at laboratories where there is proven capacity to conduct molecular testing

Resistance Vs Gene Mutation:

  • Rifampicin : rPOB
  • Isoniazid : KatG & inhA gene
  • Fluroquinolones : gyra gene
  • Aminoglycosides : rrs gene
  • Cyclic peptides : rrs gene
  • Ethambutol : embB gene 

Conclusion:

We recommend Hain test to diagnose MDR-TB in highly suspected cases, and to wait for conventional culture results to confirm NTM(Non-tuberculous Mycobacteria). Hain test has benefit in negative direct smear

References: 

1. www.ncbi.nlm.nih.gov
2.  https://www.tbfacts.org/drug-susceptibility/
3. "Molecular Line-Probe Assay for the Detection of Resistance to Second Line Anti-TB Drugs”, WHO, www.who.int/tb/areas-of-work/laboratory/policy_statements

Comments

  1. Jhon mac30 July 2019 at 11:17

    I'm glad you enjoyed the post.Keep sharing such useful informations.So informative blog, thanks for providing valuable information. Thanks for your great and helpful presentation I like your good service.I always appreciate your post.
    Gene assay

    ReplyDelete

Post a Comment

Popular posts from this blog

Chhap Tilak Sab Chhini Re Song | Lyrics in Hindi with Meaning in English

Introduction:  Basically this is poem written by Amir Khusro in 14th Century.  छाप तिलक सब छीनी रे मोसे नैना मिलाइके You've taken away my looks, my identity, by just a glance. बात अगम कर दिनी रे तो से नैना मिलाई के You've said the unknown, by just a glance. प्रेम भटी का मदवा पिलाइके By making me drink the wine from the love of devotion मतवारी कर लीन्ही रे मोसे नैना मिलाइके You've intoxicated me by just a glance; गोरी गोरी बईयाँ, हरी हरी चूड़ियाँ My fair, delicate wrists with green bangles in them बईयाँ पकड़ धर लीन्ही रे मोसे नैना मिलाइके Have been taken off by you with just a glance. बल बल जाऊं मैं तोरे रंग रजवा I give my life to you, Oh my cloth-dyer, अपनी सी रंग दीन्ही रे मोसे नैना मिलाइके You've dyed me like yourself, by just a glance. खुसरो निजाम के बल बल जाए I give my whole life to you Oh, Nizam, मोहे सुहागन कीन्ही रे मोसे नैना मिलाइके You've made me your bride, by just a glance. छाप तिलक सब छीनी रे मोसे नैना मिलाइके You've taken
1 comment
Read more

Dil Hoom Hoom Kare Song | Lyrics in Hindi with Meaning in English

Introduction: Basically this song lyrics written by Gulzar; all music composed by Bhupen Hazarika. Gulzar दिल हूम हूम करे, घबराए Dil hoom hoom kare, ghabraaye (The heart rumbles and mumbles) घन धम धम करे, गरजाए Ghan dham dham kare, darr jaaye (dark clouds of worries roar and thunder) एक बूँद कभी पानी की, मोरे अँखियों से बरसाए Ek boond kabhi paani ki mori ankhiyon se barsaaye (and yet I keep yearning, for even a single drop of tears to burst forth from my eyes) तेरी झोली डारूँ, सब सूखी पात जो आए Teri jhori daaroon sab sukhe paat jo aaye (I know your touch can sprout life in these shrivelled stumps of my existence) तेरा छुआ लगे, मेरी सूखी डाल हरियाए Tera chhua laage, meri sukhi daar hariyaaye (and in this hope I gather and preserve all the wilted leaves of my life) Statue of Bhupen Hazarika दिल हूम हूम करे, घबराए Dil hoom hoom kare, ghabraaye (The heart rumbles and mumbles) जिस तन को छूआ तूने उस तन को छुपाऊँ Jis tan ko chhua tune, us tan ko chhupaa
2 comments
Read more

Na Daro Har So Jab Jaye Lado Song | Lyrics in Hindi with Meaning in English

Introduction: Original name of this song is Deh Shiva Bar Mohe. Originally this is 17th-century hymn( भजन)  written by Guru Govind Singh(10th Sikh Guru) in the Dasam Granth in Brij Bhasha language. न डरौं अरि सौं जब जाय लड़ौं, निश्चय कर अपनी जीत करौं, May I battle all foes ( दुश्मनों)  without fear, and claim victory with courage, न डरौं अरि सौं जब जाय लड़ौं, निश्चय कर अपनी जीत करौं, May I battle all foes without fear, and claim victory with courage, निश्चय कर अपनी जीत करौं, Claim victory with courage, देह शिवा बर मोहे ईहे, शुभ कर्मन ते कभुं न टरूं, Grant me this boon( वरदान) , O God | May I never refrain from righteous acts, देह शिवा बर मोहे ईहे, शुभ कर्मन ते कभुं न टरूं, Grant me this boon( वरदान) , O God | May I never refrain from righteous acts, अरु सिख हों आपने ही मन कौ इह लालच हउ गुन तउ उचरों, May the glory be etched on my mind, And my highest goal be singing the praise, जब आव की अउध निदान बनै अति ही रन मै तब जूझ मरों, When this moral life comes to an e
2 comments
Read more